NCL Method ITA-31
Detection of Nanoparticle-Mediated Total Oxidative Stress in T-Cells Using CM-H2DC-FDA Dye
Listed in Datasets | publication by group NCL Protocols
Version 1.0 - published on 04 May 2020 doi:10.17917/PRQZ-3095 - cite this
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Description
The purpose of this protocol is to understand the propensity of nanomaterials to induce oxidative stress. Induction of oxidative stress is a common toxicity associated with nanoparticles. Generation of reactive oxygen species (ROS) and its control by antioxidant systems is a part of normal cellular metabolism [1]. However, disturbance of redox homeostasis and accumulation of ROS damages cellular organelles and DNA, eventually leading to cell death [2]. Another consequence of excessive ROS generation is activation of stress-related pathways and release of pro-inflammatory cytokines [1].
Content List
- NCL_Method_ITA-31_Feb2019.pdf(PDF | 503 KB)
Cite this work
Researchers should cite this work as follows:
- Ankit Shah, Marina Dobrovolskaia (2020). NCL Method ITA-31. NCI Hub. doi:10.17917/PRQZ-3095
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NCL Protocols
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