NCL Method GTA-1
This protocol describes the cytotoxicity testing of nanoparticle formulations porcine proximal tubule cells (LLC-PK1), as part of the in vitro NCL preclinical characterization cascade.
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Version 1.0 - published on 09 Mar 2020 doi:10.17917/5S8G-WB35 - cite this Last public release: 2.0b
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Description
This protocol describes the cytotoxicity testing of nanoparticle formulations porcine proximal tubule cells (LLC-PK1), as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes two methods for estimation of cytotoxicity, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) reduction and lactate dehydrogenase (LDH) release.
Content List
- NCL_Method_GTA-1_Nov 2015.pdf(PDF | 172 KB)
Cite this work
Researchers should cite this work as follows:
- Stern, S., Adiseshaiah, P., Potter, T. (2020). NCL Method GTA-1. NCI Hub. doi:10.17917/5S8G-WB35
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NCL Protocols
This publication belongs to the NCL Protocols group.
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