NCL Method GTA-1

By Stephan Stern1, Pavan Adiseshaiah1, Timothy Potter1

Leidos Biomedical Research, Inc.

This protocol describes the cytotoxicity testing of nanoparticle formulations porcine proximal tubule cells (LLC-PK1), as part of the in vitro NCL preclinical characterization cascade.

Listed in Datasets | publication by group NCL Protocols

Version 2.0b - published on 09 Jul 2020 doi:10.17917/Q6EC-XC97 - cite this

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Description

This protocol describes the cytotoxicity testing of nanoparticle formulations porcine proximal tubule cells (LLC-PK1), as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes two methods for estimation of cytotoxicity, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) reduction and lactate dehydrogenase (LDH) release.

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