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Baylor Residual breast cancer

By Creighton C1, Lewis M1, Chang J1, Jonathan Bistline2

1. Baylor College of Medicine 2. Broad Institute

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Abstract

Residual breast cancers after conventional therapy display mesenchymal as tumor-initiating features.  There are four (4) files included in this study:

  1. Sample Annotation (combined_sample_annotation.txt)
  2. Gene expression data from CD44+/CD24- cells sorted by flow cytometry (GSE7513.gct)
    Human breast tumor samples were sorted using flow cytometry to select for cells that were CD44+ and CD24-. Gene expression profiles of these cells were compared with profiles of the other sorted cells (CD24+ and CD44-/CD24-).  GEO Accession GSE7513.
  3. Gene expression data from cancer mammospheres and bulk tumors (GSE7515.gct)
    Isolated single cell suspensions from primary breast cancers were plated onto non-adherent (polyhema-coated) plastic, counted with a hematocytometer, and 20,000 cells were then seeded into a 6-well ultra-low attachment plate supplemented with 2mL MEGM, with the addition of 2 mL of freshly unfrozen MEGM every 3-4 days. Gene expression profiles were taken of both MS and primary bulk tumors and compared with each other.  GEO Accession: GSE7515
  4. Letrozole (Femara) early response to treatment (GSE10281.gct)
    Biopsies were taken from the same subjects both pretreatment and after 10-14 days Letrozol, 2.5 mg/day, oral.  GEO Accession GSE10281

These datasets have been exported from the Broad Institute's ICBP Data & Analysis Portal.

Credits

Creighton C, Lewis M, Chang J

Sponsored by

Baylor College of Medicine, Broad Institute of MIT & Harvard

Cite this work

Researchers should cite this work as follows:

  • Creighton C; Lewis M; Chang J; Jonathan Bistline (2014), "Baylor Residual breast cancer," https://ncihub.org/resources/617.

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