Advances in the reliability of nanotoxicity assays

By Elijah Petersen

NIST

Download (PDF)

Licensed under

Published on

Abstract

Nano WG February 20, 2020

Abstract: With the increasing usage of nanotechnology in consumer products, it is important to develop accurate methods to assess the potential adverse effects of engineered nanomaterials (ENMs) on humans or environmentally important organisms. However, many of these measurements are challenged by the unique behaviors of ENMs which differ from those of dissolved metals or hydrophobic organic chemicals. Thus, modifications to typical toxicity assays are needed to account for these behaviors to avoid artifacts and ensure accurate measurements. In this talk, I will first discuss the different artifacts that can occur during measurements on the toxicological effects of nanomaterials and potential control experiments to assess these artifacts. Then, I will discuss the usage of measurement science tools, such as cause-and-effect analysis and incorporation of process control measurements into the assay plate design, to support the successful interlaboratory comparison of the MTS nanocytotoxicity assay, and the evaluation of an ecotoxicity assay, an International Standardization Organization C. elegans growth and reproduction inhibition assay, for use with nanomaterials.

  

Cite this work

Researchers should cite this work as follows:

  • Elijah Petersen (2020), "Advances in the reliability of nanotoxicity assays," https://ncihub.org/resources/2319.

    BibTex | EndNote

Submitter

Mervi Heiskanen

National Cancer Institute

Tags