Identification of the key node in the expression network of BLM, EXO1, and DNA2 in Homologous Repair mediated DNA damage response
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Abstract
DNA2, EXO1, and BLM are known to be a part of the end resection rate limiting step of the Homologous Recombination (HR) DNA damage repair (DDR) pathway. Inhibition of these three proteins would in effect inhibit the HR repair pathway. In cancer cells where the HR repair pathway is hyperactivated there is increased risk of metastasis, decreased patient survival rates and chemotherapy resistance. Inhibition of this pathway could lead to a decrease of these side-effects. In this experiment, I tried to establish cell lines that overexpress the DNA2, BLM, and EXO1 proteins in order to do a transcriptome analysis on them and be able to identify common upregulated genes that can be targeted using drugs in order to reverse the chemotherapy resistance in tumors that have uncontrolled HR response. Due to time limitations and the need for protocol troubleshooting, I was not able to finish establishing the cell lines.
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This presentation is supported by the National Cancer Institute through the U54 CA096297/CA096300: UPR/MDACC Partnership for Excellence in Cancer Research Training Program.
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