NCL Method STE-1.3
Detection and Quantification of Gram-Negative Bacterial Endotoxin Contamination in Nanoparticle Formulations Using the Gel-Clot LAL Assay
Listed in Datasets | publication by group NCL Protocols
Version 1.0 - published on 14 Jun 2021 doi:10.17917/VRHF-5Y74 - cite this
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Description
This document discusses the quantitative detection of Gram-negative bacterial endotoxin in nanoparticle preparations using the gel-clot Limulus Amebocyte Lysate (LAL). The protocol for this assay is based on instructions provided with the reagents from Associates of Cape Cod as well as the USP standard 85 “Bacterial endotoxin test” [1]. In lieu of detailing the exact procedure here (which can be found in reference [1], a “Bench Sheet” is provided that can be used in conjunction with the USP protocol.
Content List
- NCL_Method_STE-1.3_2020.pdf(PDF | 486 KB)
Cite this work
Researchers should cite this work as follows:
- Barry Neun, Marina Dobrovolskaia (2021). NCL Method STE-1.3. NCI Hub. doi:10.17917/VRHF-5Y74
NCL Protocols
This publication belongs to the NCL Protocols group.
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