NCL Method ITA-9.2
In vitro Assay for Assessing Nanoparticle effects on Monocyte/Macrophage Phagocytic Function
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Version 1.0 - published on 09 Jul 2020 doi:10.17917/RXJ2-EG43 - cite this Last public release: 2.0
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Description
This document describes a protocol for evaluation of nanoparticle effects on the phagocytic function of the immune cells. Phagocytosis is a receptor-mediated endocytosis peculiar to the phagocytic cells, e.g. cells of the mononuclear phagocytic system (MPS). Phagocytosis is an active process and requires actin polymerization. There are four primary receptors which mediate phagocytic uptake. Phagocytosis via three of these receptors (complement receptor (CR), FcgR receptor, and mannose receptor (MR)) is accompanied by inflammatory reactions (cytokine secretion). Phagocytosis via the fourth receptor (scavenger receptor (SR)) is not accompanied by inflammatory responses (1-5).
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NCL_Method_ITA-9.2_MAy2020.pdf(PDF | 398 KB)
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Researchers should cite this work as follows:
- Timothy Potter, Barry Neun, Edward Cedrone, Marina Dobrovolskaia (2020). NCL Method ITA-9.2. NCI Hub. doi:10.17917/RXJ2-EG43
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NCL Protocols
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