This document describes a protocol for assessing the effect of a nanoparticle formulation on the basic immunologic function of human lymphocytes, i.e. measurement of lymphocyte proliferative responses (1, 2). This assay will allow for measurement of a nanoparticles’ ability to induce proliferative response of human lymphocytes (part A) or to suppress that induced by phytohemaglutinin (PHA-M) (part B).   Parts A and B can typically be run concurrently on the same test plate.  Part A is also available as a separate protocol entitled ITA-6.2.  Part B is available as a separate protocol with a modification to allow for 24 h exposure to nanoparticle prior to challenge with PHA-M, and is entitled ITA-6.3.

• NCL_Method_ITA-6.1_ May2020.pdf(PDF | 370 KB)

Researchers should cite this work as follows:

• Timothy Potter, Edward Cedrone, Sarah Skoczen, Barry Neun, Marina Dobrovolskaia (2020). NCL Method ITA-6.1. (Version 2.0). NCI Hub. doi:10.17917/C8XW-BQ05

  BibTex | EndNote

1. leukocyte proliferation
2. Nanoparticles