NCL Method IEA-1
Analysis of nanoparticle effects on invasion of cancer cells
Listed in Datasets | publication by group NCL Protocols
Version 2.0 - published on 09 Jul 2020 doi:10.17917/GS4C-N171 - cite this
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Description
This document provides a protocol for a real-time, quantitative assessment of nanoparticles-mediated inhibition of cancer cell invasion. Although processes of cell migration and invasion have similar properties, they can be differentiated based on the ability of invasive cells to migrate through either an extracellular matrix (ECM) or a basement membrane extract (BME). This protocol uses Matrigel as an extracellular matrix and analyzes the number of cells migrating through the Matrigel and porous membrane barriers with or without prior exposure to test nanoparticles. Nanoparticles, which intended mechanism of action involves the inhibition of cancer cell invasion, will result in a decrease in the number of cells migrated through these barriers.
Content List
NCL_Method_IEA-1_May2020.pdf(PDF | 647 KB)
Cite this work
Researchers should cite this work as follows:
- Julia Bui, Edward Cedrone, Naichen Yu, Marina Dobrovolskaia (2020). NCL Method IEA-1. (Version 2.0). NCI Hub. doi:10.17917/GS4C-N171
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NCL Protocols
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