NCL Method PCC-19
Asymmetric-flow field-flow Fractionation
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Version 2.0 - published on 09 Jul 2020 doi:10.17917/8S1D-BN17 - cite this
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Description
Nanomaterials are inherently polydispersed. Traditional techniques, such as the widely used batch-mode dynamic light scattering (DLS) analysis, are not ideal nor thoroughly descriptive enough to define the full complexity of these materials. Asymmetric-flow field-flow fractionation (AF4) with various in-line detectors, such as ultraviolet–visible (UV-Vis), multi-angle light scattering (MALS), refractive index (RI) and dynamic light scattering (DLS) is an alternative technique that can provide flow-mode analysis of not only size distribution, but also shape, drug release/stability, and protein binding [1-3].
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NCL_Method_PCC-19_May2020.pdf(PDF | 612 KB)
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Researchers should cite this work as follows:
- Jeffrey Clogston, Yingwen Hu (2020). NCL Method PCC-19. (Version 2.0). NCI Hub. doi:10.17917/8S1D-BN17
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NCL Protocols
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