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NCL Method STE-2.2

By Timothy Potter, Barry Neun, Anna Ilinskaya, Marina Dobrovolskaia

Detection of Bacterial Contamination by Agar Plate Test

Listed in Datasets | publication by group NCL Protocols

Version 1.0 - published on 04 May 2020 doi:10.17917/5JCE-HQ72 - cite this

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Description

This protocol describes a procedure for quantitative determination of microbial contamination in a nanoparticle preparation. Nanoparticle samples along with controls are spread on the surface of agar, and growth of bacterial colonies is monitored after 72 hr incubation.

Note: This assay is not intended to certify the material as sterile. The intended purpose is to avoid introduction of microbial contamination into in vitro cell cultures and in vivo animal studies utilizing the test-nanomaterial, as microbial contamination will confound the results of these tests. 

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