NCL Method STE-1.3
Detection and Quantification of Gram Negative Bacterial Endotoxin Contamination in Nanoparticle Formulations by Gel-Clot LAL Assay
Listed in Datasets | publication by group NCL Protocols
Version 1.0 - published on 04 May 2020 doi:10.17917/VZ9M-8351 - cite this
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Description
This document discusses the quantitative detection of Gram negative bacterial endotoxin in nanoparticle preparations using the gel-clot Limulus Amebocyte Lysate (LAL) assay. The protocol for this assay is based on instructions provided with the reagents from Associates of Cape Cod as well as USP standard 85, “Bacterial endotoxin test [1]. In lieu of detailing the exact procedure here (which can be found in reference [1], a “Bench Sheet” is provided that can be used in conjunction with the USP protocol.
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NCL_Method_STE-1.3_Sep2015.pdf(PDF | 158 KB)
Cite this work
Researchers should cite this work as follows:
- Barry Neun, Anna Ilinskaya, Marina Dobrovolskaia (2020). NCL Method STE-1.3. NCI Hub. doi:10.17917/VZ9M-8351
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NCL Protocols
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