NCL Method STE-1.1

By Barry Neun, Anna Ilinskaya, Marina Dobrovolskaia

Detection and Quantification of Gram Negative Bacterial Endotoxin Contamination in Nanoparticle Formulations by End Point Chromogenic LAL Assay

Listed in Datasets | publication by group NCL Protocols

Version 1.0 - published on 04 May 2020 doi:10.17917/A20K-T950 - cite this

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Description

This document describes a protocol for a quantitative detection of Gram negative bacterial endotoxin in nanoparticle preparations using an end-point Limulus Amebocyte Lysate (LAL) assay. The protocol is based on the QCL-1000 kit manufactured by Lonza Corporation [1] and USP standard 85, “Bacterial endotoxin test” [2].

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