NCL Method ITA-25
Enzyme-Linked Immunosorbent Assay (ELISA) for detection of human IFN in culture supernatants
Listed in Datasets | publication by group NCL Protocols
Version 2.0 - published on 09 Jul 2020 doi:10.17917/ZNS7-4E84 - cite this Last public release: 2.0b
Licensed under these terms
Description
IFNg is type II interferon produced by lymphocytes (T-cells, NK cells, NKT cells) in response to viral infections and inflammatory stimuli, including but not limited to bacterial lipopolysaccharide LPS and CpG DNA. Factors mitogenic to T-cells (e.g. PHA-M) can also induce this interferon. IFNg is essential in maintaining function of adaptive immunity through regulation of T cell function. It is also important for protection from intracellular bacteria (e.g. M.tuberculosis) and anti-tumor immunity. This document describes experimental procedure for analysis of culture supernatants by ELISA to detect presence of IFNg. NCL protocol ITA-10 should be referred to for details of preparation of culture supernatants.
Content List
NCL_Method_ITA-25_June2020.pdf(PDF | 553 KB)
Cite this work
Researchers should cite this work as follows:
- Edward Cedrone, Timothy Potter, Barry Neun, Marina Dobrovolskaia (2020). NCL Method ITA-25. (Version 2.0). NCI Hub. doi:10.17917/ZNS7-4E84
Tags
NCL Protocols
This publication belongs to the NCL Protocols group.
When watching a publication, you will be notified when a new version is released.