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NCL Method ITA-25

By Barry Neun, Jamie Rodriguez, Timothy Potter, Anna Ilinskaya, Marina Dobrovolskaia

Enzyme-Linked Immunosorbent Assay (ELISA) for detection of human IFNγ in culture supernatants

Listed in Datasets | publication by group NCL Protocols

Version 1.0 - published on 04 May 2020 doi:10.17917/ZXVG-DA66 - cite this Last public release: 2.0b

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Description

IFNg is type II interferon produced by lymphocytes (T-cells, NK cells, NKT cells) in response to viral infections and inflammatory stimuli, including but not limited to bacterial lipopolysaccharide (LPS) and CpG DNA. Factors mitogenic to T-cells (e.g., PHA-M) can also induce this interferon. IFNg is essential in maintaining the function of adaptive immunity through regulation of T cell function. It is also important for protection from intracellular bacteria (e.g., M.tuberculosis) and anti-tumor immunity. This document describes an experimental procedure for analysis of cell culture supernatants for the presence of IFNg using an enzyme linked immunosorbent assay (ELISA). Refer to NCL protocol ITA-10 for details regarding culture supernatant preparation.

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