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NCL Method ITA-24

By Barry Neun, Jamie Rodriguez, Timothy Potter, Anna Ilinskaya, Marina Dobrovolskaia

Enzyme-Linked Immunosorbent Assay (ELISA) for detection of human TNF-α in culture supernatants

Listed in Datasets | publication by group NCL Protocols

Version 1.0 - published on 04 May 2020 doi:10.17917/X900-GP60 - cite this Last public release: 2.0b

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Description

TNF-a is a pro-inflammatory chemokine produced by various types of cells in response to inflammatory stimulus, e.g., bacterial lipopolysaccharide (LPS). This document describes an experimental procedure for analysis of cell culture supernatants for the presence of TNF-a using an enzyme linked immunosorbent assay (ELISA). Refer to NCL protocol ITA-10 for details regarding culture supernatant preparation.

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