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NCL Method ITA-22

By Barry Neun, Jamie Rodriguez, Timothy Potter, Anna Ilinskaya, Marina Dobrovolskaia

Enzyme-Linked Immunosorbent Assay (ELISA) for detection of human IL-8 in culture supernatants

Listed in Datasets | publication by group NCL Protocols

Version 2.0b - published on 24 Jul 2020 doi:10.17917/SJT2-7P72 - cite this

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Description

CXCR8/IL-8 is a pro-inflammatory chemokine produced by various types of cells in response to inflammatory stimulus, e.g., bacterial lipopolysaccharide (LPS). This document describes an experimental procedure for analysis of cell culture supernatants for the presence of IL-8 using an enzyme linked immunosorbent assay (ELISA). Refer to NCL protocol ITA-10 for details regarding culture supernatant preparation.

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