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NCL Method ITA-22

By Edward Cedrone, Timothy Potter, Barry Neun, Marina Dobrovolskaia

Enzyme-Linked Immunosorbent Assay (ELISA) for detection of human IL-8 in culture supernatants

Listed in Datasets | publication by group NCL Protocols

Version 2.0 - published on 09 Jul 2020 doi:10.17917/EWMJ-D959 - cite this Last public release: 2.0b

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Description

CXCR8/IL-8 is a proinflammatory chemokine produced by various types of cells in response to inflammatory stimulus, e.g. bacterial lipopolysaccharide LPS. This document describes experimental procedure for analysis of cell culture supernatants for the presence of IL-8 by enzyme linked immunosorbent assay (ELISA).

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