NCL Method ITA-18
Human Leukocyte Proliferation Assay (HuLa)
Listed in Datasets | publication by group NCL Protocols
Version 2.0b - published on 24 Jul 2020 doi:10.17917/416C-5429 - cite this
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Description
Immunosuppression is a common reason for drug withdrawal from the market. Traditional immune function tests used to estimate a material’s immunosuppression is T-cell dependent antibody response (TDAR). This method involves a 28 day in vivo study evaluating an animal’s antibody titer to a known antigen (KLH) with and without challenge. Due to the limited quantities of novel drug candidates, an in vitro method called Human Leukocyte activation (HuLa) has been developed as a substitute for the traditional TDAR assay during early preclinical evaluation. The method presented herein is based on the HuLa assay originally developed by Mark Collinge, et al at Pfizer [1]. The protocol presented in this document has been adapted for use with engineered nanomaterials.
Content List
- NCL_Method_ITA-18_June2020.pdf(PDF | 409 KB)
Cite this work
Researchers should cite this work as follows:
- Timothy Potter, Barry Neun, Sarah Skoczen, Anna Ilinskaya, Marina Dobrovolskaia (2020). NCL Method ITA-18. (Version 2.0b). NCI Hub. doi:10.17917/416C-5429
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NCL Protocols
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