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NCL Method ITA-12

By Edward Cedrone, Timothy Potter, Barry Neun, Marina Dobrovolskaia

In vitro Analysis of Nanoparticle Effects on Plasma Coagulation Time

Listed in Datasets | publication by group NCL Protocols

Version 2.0 - published on 09 Jul 2020 doi:10.17917/GV9W-FF90 - cite this Last public release: 2.0b

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Description

This document describes a protocol for assessing the effect a nanoparticle formulation may have on plasma coagulation time. Coagulation, i.e., blood clotting, is a highly complex process that involves many components. There are three main pathways for coagulation: intrinsic (also known as the contact activation pathway, because it is activated by a damaged surface); extrinsic (also known as the tissue factor pathway); and the final common pathway. Each pathway can be assessed by a specialized test. For example, the activated partial thromboplastin time (APTT) assay is used to assess the intrinsic pathway, while the prothrombin time (PT) assay is a measure of the extrinsic pathway. Extrinsic and intrinsic pathways converge into common pathway. Thrombin time (TT) is an indicator of the functionality of the final common pathway. Each pathway involves many coagulation factors, some of which overlap between pathways. The APTT assay assesses functionality of factors XII, XI, IX, VIII, X, V, II. The PT assay assesses activity of factors VII, X, V and II. All three assays assess the role of fibrinogen.

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