NCL Method ITA-8

By Sarah Skoczen, Barry Neun, Timothy Potter, Jamie Rodriguez, Anna Ilinskaya, Marina Dobrovolskaia

Chemotaxis Assay

Listed in Datasets | publication by group NCL Protocols

Version 1.0 - published on 04 May 2020 doi:10.17917/W604-T506 - cite this

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This document provides a protocol for rapid, quantitative measure of the chemoattractant capacity of a nanoparticulate material. Leukocyte recruitment is a central component of the inflammatory process, both in physiological host defense and in a range of prevalent disorders with an inflammatory component. In response to a complex network of pro-inflammatory signaling molecules (including cytokines, chemokines and prostaglandins), circulating leukocytes migrate from the bloodstream to the site of inflammation. 

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