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NCL Method ITA-6

By Timothy Potter, Barry Neun, Sarah Skoczen1, Jamie Rodriguez, Anna Ilinskaya, Marina Dobrovolskaia

Leidos Biomedical Research, Inc.

Leukocyte Proliferation Assay

Listed in Datasets | publication by group NCL Protocols

Version 1.0 - published on 04 May 2020 doi:10.17917/4VDA-ZS32 - cite this

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Description

This document describes a protocol for assessing the effect of a nanoparticle formulation on the basic immunologic function of human lymphocytes, i.e. measurement of lymphocyte proliferative responses [1, 2]. This assay will allow for measurement of a nanoparticles’ ability to induce proliferative response of human lymphocytes or to suppress that induced by phytohemaglutinin (PHA-M).

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