Proteins bind the surfaces of nanoparticles, and biological materials in general, immediately upon introduction into a physiological environment. The further biological response of the body is influenced by the nanoparticle–protein complex. The nanoparticle's composition and surface chemistry dictate the extent and specificity of protein binding. In general, charged particles bind more proteins than their neutral counterparts. Protein binding is one of the key elements that effect particle uptake by the phagocytic cells of the immune system, and therefore, the biodistribution of the nanoparticles throughout the body (Figure 1) [1-4]. Protein binding may or may not affect the activity of the protein in the “corona” surrounding the nanoparticle [5]. As such, using protein binding in lieu of specialized immunotoxicity assays is not recommended.

• NCL_Method_ITA-4_May2020.pdf(PDF | 681 KB)

Researchers should cite this work as follows:

• Barry Neun, Chris McLeland, Timothy Potter, Anna Ilinskaya, Marina Dobrovolskaia, Suzanne Specht, Tad Guszcynski (2020). NCL Method ITA-4. (Version 2.0b). NCI Hub. doi:10.17917/822G-S949

  BibTex | EndNote

1. Nanoparticles
2. protein corona