NCL Method GTA-7

By Stephan Stern, Banu Zolnik1

Leidos Biomedical Research, Inc.

Hepatocyte Primary ROS Assay

Listed in Datasets | publication by group NCL Protocols

Version 2.0 - published on 09 Jul 2020 doi:10.17917/0WF6-GX40 - cite this

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Description

This protocol describes the testing of nanoparticle formulations for reactive oxygen species (ROS) generation in male Sprague-Dawley (SD) primary hepatocytes, as part of the in vitro NCL preclinical characterization cascade.  The protocol utilizes a fluorescent redox active probe.  Primary hepatocytes were chosen since they have a greater metabolic activity than hepatocyte cell lines.

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