NCL Method GTA-6
HepG2 Hepatocarcinoma Apoptosis Assay
Listed in Datasets | publication by group NCL Protocols
Version 1.0 - published on 09 Mar 2020 doi:10.17917/RJYY-4562 - cite this Last public release: 2.0
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Description
This protocol describes the monitoring of nanoparticle treated human hepatocarcinoma cells (HepG2) for apoptosis, as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes a fluorescent method to determine the degree of caspase-3 activation.
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- NCL_Method_GTA-6.pdf(PDF | 427 KB)
Cite this work
Researchers should cite this work as follows:
- Stephan Stern, Timothy Potter (2020). NCL Method GTA-6. NCI Hub. doi:10.17917/RJYY-4562
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The publication is part of the series below:
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Effect of blood collection tube type and time to assay on the enumeration of circulating tumor cells using the High Definition Single Cell Analysis (HD-SCA) method, laboratory of Peter Kuhn Ph.D.
The goal of this study is to characterize the effect of two pre-analytical variables on the enumeration of circulating tumor cells using the High Definition Single Cell Analysis assay. The optimized assay was then compared to the CellSearch® System.
NCL Protocols
This publication belongs to the NCL Protocols group.
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