NCL Method GTA-3

By Stephan Stern, Timothy Potter, Barry Neun1

Leidos Biomedical Research, Inc.

Hep G2 Hepatocyte Glutathione Assay

Listed in Datasets | publication by group NCL Protocols

Version 2.0b - published on 09 Jul 2020 doi:10.17917/EHR9-HB86 - cite this

Licensed under these terms

Description

This protocol describes the analysis of human hepatocarcinoma cells (Hep G2) for reduced and oxidized glutathione, following treatment with nanoparticle formulations, as part of the NCL in vitro preclinical characterization cascade.  A shift from reduced to oxidized glutathione is indicative of oxidative stress, while a decrease in the overall reduced glutathione pool is indicative of conjugative metabolism or impaired synthesis (1). This protocol utilizes a colorimetric method for glutathione determination.

Content List

Cite this work

Researchers should cite this work as follows:

Tags

Recommendations

Powered by ...

NCL Protocols

NCL Protocols group image

When watching a publication, you will be notified when a new version is released.