NCL Method GTA-2

By Stephan Stern, Pavan Adiseshaiah, Timothy Potter

Hep G2 Hepatocarcinoma Cytotoxicity Assay

Listed in Datasets | publication by group NCL Protocols

Version 2.0b - published on 09 Jul 2020 doi:10.17917/96Z2-NZ61 - cite this

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Description

This protocol describes the cytotoxicity testing of nanoparticle formulations in human hepatocarcinoma cells (Hep G2), as part of the in vitro NCL preclinical characterization cascade. The protocol utilizes two methods for estimation of cytotoxicity, 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) reduction and lactate dehydrogenase (LDH) release.

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