The IMPC is generating a knockout mouse strain for every protein coding gene by using the embryonic stem cell resource generated by the International Knockout Mouse Consortium (IKMC). The production of mouse strains from these ES cells are tracked within the "international Micro-injection tracking system" (iMits) and are made available to the research community via public repositories.

Systematic broad-based phenotyping is performed by each IMPC center using standardized procedures found within the International Mouse Phenotyping Resource of Standardised Screens (IMPReSS) resource. Gene-to-phenotype associations are made by a versioned statistical analysis with all data freely available by this web portal and by several data download features.