__'''PSON0001 Genomic Characterization of Cell Lines'''__ This study provides genomic characterization of 40 cell lines from Physical Sciences Oncology Center (PS-OC) Network Bioresource Core Facility (PBCF) through the sequencing of mRNA (TruSeq Stranded RNA Sample Preparation Kit), miRNA (TruSeq Small Total RNA Sample Prep Kit ) and exome (Agilent SureSelectXT2 Human All Exon V5 kit) and aligning to a reference genome (HG19/37 ). Software for the mRNA characterizations included: cufflinks, cuffdecoder, fastcqReports, mapsplice, tophat, transdecoder, for miRNA included: bowtie2, miRanalyzer, miTools. and for exome included bowtie2. Datasets include the output specific to these software packages, as well as more widely used bioinformatics formats: bam, bed, fasta, fastq, vcf (4.1). Download the dataset at [ftp://caftpd.nci.nih.gov/psondcc/CellLineGenomics/]